Short CommunicationCutaneous infection with Dermocystidium salmonis in cardinal tetra, Paracheirodon axelrodi (Schultz, 1956)M C Langenmayer1, E Lewisch2, M Gotesman2, W Hoedt3, M Schneider3, M El-Matbouli2 and W Hermanns11 Institute of Veterinary Pathology at the Centre for Clinical Veterinary Medicine, LMU Munich, Munich, Germany2 Clinical Division of Fish Medicine, University of Veterinary Medicine, Vienna, Austria 3 Veterinary Practice Dr W Hoedt, Rosenheim, GermanyKeywords: cardinal tetra, Dermocystidium salmonis, electron microscopy, histology, Paracheirodonaxelrodi. The genus Dermocystidium comprises fungalpathogens of the order Dermocystida in the class Ichthyosporea (http://www.ncbi.nlm.nih.gov/taxonomy).Several different Dermocystidium species have been described, infecting a variety of fishhosts and producing gill infections, skin lesions and visceral disease all over the world (Wildgoose1995; Pekkarinen & Lotman 2003; Pekkarinen et al. 2003; Feist et al. 2004; Zhang & Wang2005). To our knowledge, there are two reports on Dermocystidium infections in cardinal tetra,Paracheirodon axelrodi and neon tetra, Paracheirodon innesi (Reichenbach-Klinke 1982; Lewisch2010). However, these reports did neither include histological and ultrastructural examinations normolecular genetic investigations to confirm the diagnosis and identify the aetiologic species.In January 2013, increased mortalities of cardinal tetra, P. axelrodi of a 350-L aquarium werereported, occurring after purchase of additional fish. During the clinical examination, most of thefish were swimming in normal active condition, but some were lethargic or displayed a transparentmass on the skin of the head or body. The masses were up to 5 mm in diameter and contained acentral, white tubular structure. Similar masses were located on the fins of some fish, but wereconsiderably smaller in these locations. A parasitic infestation of the skin of these fish was excludedvia microscopic examination, and normal results were obtained after analysis of the water valuesand inspection of the pump and filter system. Six cardinal tetra, P. axelrodi and two fireheadtetra, Hemigrammus bleheri were submitted for pathological examination in formalin and formolecular genetic examination in ethanol. Only two cardinal tetra displayed skin lesions. Beforeembedding, one tetra was post-fixed in Davidson’s fixative and sections of glycolmethacrylate/methylmethacrylate-embedded samples were routinely processed for histological examination and stainedwith haematoxylin and eosin (HE), Giemsa, silver impregnation and periodic acid Schiff (PAS) reactionaccording to standard protocols. Samples of the mass were also routinely processed for transmissionand scanning electron microscopy on a transmission electron microscope (Zeiss EM 10)or on a digital scanning electron microscope (Zeiss DSM 950), respectively.The macroscopic examination of the cardinal tetra revealed a focally extensive, bulging, hemispherical,transparent oedema (4 mm in diameter) of the ventral skin on the head. Central, withinCorrespondence M C Langenmayer, Institute of Veterinary Pathology at the Centre for Clinical Veterinary Medicine, LMUMunich, Veterin€arstrasse 13, 80539 Munich, Germany (e-mail: firstname.lastname@example.org)Langenmayer and Lewisch contributed equally to this work. 12014 John Wiley & Sons LtdJournal of Fish Diseases 2014 doi:10.1111/jfd.12281
Strain info aqui:
Se quiseres dá-me o teu mail que envio a info que tenho, que eu saiba ainda não se conhece tratamento, há uns tempos "falei" com um conhecido que testou um derivado de nistatina mas não sei resultados.
Também não me virei mais para isso... :/
PS: o link que corrijo agora...sorry! http://www.aquariofilia.net/forum/index.php?showtopic=236241&hl=